Substance P EIA Kit Application Notes

1. If not in use, kit apparatus should be refrigerated. All reagents should be broiled to allowance temperature afore use.

2. Microtiter plates should be accustomed to appear to allowance temperature afore aperture the antithesis bags. Once the adapted amount of strips has been removed, anon reseal the bag and abundance at 2-8°C to advance bowl integrity.

3. Samples should be calm in pyrogen/endotoxin-free tubes.

4. Samples should be arctic if not analyzed anon afterwards collection. Abstain assorted freeze-thaw cycles of arctic samples. Thaw absolutely and mix able-bodied above-mentioned to analysis.

5. If possible, abstain use of abominably hemolyzed or lipemic sera. If ample amounts of chapped amount are present, centrifuge or clarify above-mentioned to analysis.

6. It is recommended that all standards, controls and samples be run in duplicate.

7. If pipetting reagents, advance a constant adjustment of accession from well-to-well. This ensures according evolution times for all wells.

8. Cover or cap all reagents if not in use.

9. Do not mix or altering altered reagent lots from assorted kit lots.

10. Do not use reagents afterwards the kit cessation date.

11. Read absorbances aural 2 hours of appraisal completion.

12. If provided, controls should be run with every assay. If ascendancy ethics abatement alfresco pre-established ranges, the accurateness of the appraisal is suspect.

13. All balance ablution aqueous have to be drained from the wells by able aspiration or by decantation followed by borer the bowl angrily on absorptive paper. Never admit absorptive cardboard anon into the wells.

14. Because Stabilized Chromogen is ablaze sensitive, abstain abiding acknowledgment to light. Also abstain acquaintance amid Stabilized Chromogen and metal, contrarily blush may develop.

15. Incomplete abrasion will abnormally affect the analysis outcome. All abrasion have to be performed with Ablution Band-aid provided.

16. Abrasion can be performed manually as follows: absolutely aspirate the aqueous from all wells by acclaim blurred an aspiration tip into the basal of anniversary well. Take affliction not to blemish the central of the well.

17. Afterwards aspiration, ample the wells with at atomic 0.4 mL of adulterated ablution solution. Let absorb for 15 to 30 seconds, and again aspirate the liquid. Repeat as directed beneath ASSAY PROCEDURE. Afterwards the abrasion procedure, the bowl is astern and broke dry on absorptive tissue.

18. Alternatively, the ablution band-aid may be put into a eject bottle. If a eject canteen is used, flood the bowl with ablution buffer, absolutely bushing all wells.

19. If application an automatic washer, the operating instructions for abrasion accessories should be anxiously followed.

20. Appraisal Action Preliminary notes: Do not mix reagents from altered lots. It is recommended that assays be performed in duplicate. Standards and samples have to be assayed at the aforementioned time. Abstain advertisement the substrate to absolute sunlight.
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