Trichinella ELISA Kit Reagent Preparation

A) Preparation of the standard: Standard solution should be prepared no more than 2 hours prior to the experiment. Add 1 ml sample diluent buffer into one tube, dissolve the standard thoroughly and make times dilution.
B) Preparation of biotinylated anti-Human 96T.
2. Standards: 96 tests (2 vials)
3. Detection Antibody Human Trichinella antibody should be diluted in 1:99 with the antibody diluent buffer and mixed thoroughly.
C) Preparation of Avidin-Biotin-Peroxidase Complex (ABC) working solution: The solution should be prepared no more than 1 hour prior to the experiment.
a. The total volume should be: 0.1ml/well x (the number of wells). (Allowing 0.1-0.2 ml more than total volume)
b. Avidin-Biotin-Peroxidase Complex (ABC) should be diluted in 1:99 with the ABC dilution buffer and mixed thoroughly.
D) Preparation of TMB working solution: transfer 9 volumes of TMB color developing reagent A in one volume of TMB color developing reagent B for 30 minutes in 37° C before using to make TMB substrate, mixing thoroughly.
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Arg8-Vasopressin ELISA Kit

PRODUCT LINE      Signal Transduction
PRODUCT FAMILIES       Hormones & Hormone Receptors Other Products
Ordering Information
 Product Numbers   Format   Size   Unit Price   Quantity Add to Cart
ADI-901-017         5x96 wells     
Product Specification
SENSITIVITY:     3.39 pg/ml (range 4.10 - 1,000 pg/ml)
KIT/SET CONTAINS:      GxR Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
APPLICATION:       For the quantitative determination of Arg8-Vasopressin in culture supernatants from any species. Cited sample type includes plasma (ref. 1, 2).
SHIPPING:       SHIPPED ON BLUE ICE
USE/STABILITY:     Store all components at +4°, except standard and conjugate at -20°.
Product Description
The Arg8-Vasopressin EIA kit is a colorimetric competitive enzyme immunoassay kit with results overnight + 1 hour.. Absorbance is read at 405 nm. Document your results to scientists worldwide, this kit has been cited in numerous peer-reviewed journals for use in a variety of sample types including urine, perfusates, plasma, tissue, and serum. The simple liquid color-coded reagents reduce error.
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